2004-05 NCUR Travel Grant Application

Revised September 2004

IgG Gel Card Sensitivity:  The Detection of Anti-M Antibodies

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Project Title (10 words or less)

Nicole Spendlove and Zane Williams

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Student Name (last, first)

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Student ID #

                                                                                         Spring/2005

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Total Number of Credits Completed             Anticipated Graduation (term/year) *

                                                                        (*  funds may NOT be spent after graduation)

801-668-8218                                                    nicolespendlove@mail.weber.edu

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Student Phone                                                                   Student email address

   Zundel, Bill

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Faculty Mentor Name (last, first)

Clinical Laboratory Science, College of Health Professions

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Faculty Mentor Department, and College

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Student Signature                                                                      Date

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Project Mentor Signature                                                           Date

3905                                                6118

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Campus Mail Code                    Phone Extension           

 ABSTRACT

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IgG Gel Card Sensitivity:  The Detection of Anti-M with Dosage Demonstrating Red Blood Cells.

Nicole K. Spendlove and Zane H. Williams (Bill Zundel MS SBB MT (ASCP)), Department of Clinical Laboratory Science, Weber State University College of Health Professions Ogden, Ut. 84408-3905

Serum containing anti-M antibodies cross reacts with M antigen positive red blood cells, especially those cells exhibiting dosage. This reaction, if undetected in vitro may cause a hemolytic transfusion reaction in vivo.  A tube method has been primarily used to detect the presence of anti-M antibodies until recently.  New Gel Technology allows for testing standardization and increased sensitivity to antibody/antigen reactions.  Anecdotal evidence in laboratory settings suggests that gel technology is not adequate to detect the presence and reaction of anti-M antibodies especially if a dosage related reaction

occurs. In this study, traditional tube method reactions are compared to gel technology reactions. In conjunction, new and old reagent red blood cells are tested to determine gel technology sensitivity to anti-M antibodies.  Five to six anti-M positive specimens are tested against new and old, heterozygous and homozygous reagent red cells simultaneously.  Approximately 60 tests are conducted within a timeframe of several hours.  Data is collected in a blind study and interpretation is made by basis of comparison between the tube method and gel technology, then the relevance of the age of the red blood cell reagent. The study will place limitations on gel technology or resolve any doubts associated with identifying and quantifying anti-M dosage related reactions in vitro.